應用編號(Application ID):PR0048
樣品分類(Sample Category):醫(yī)藥(Pharmaceutical)
分離模式(Separation Mode):反相(Reversed phase chromatography)
產品系列(Product Line):HP
方法(Method):
Column: |
HP-C18, 5 µm, 120 Å,4.6 × 150 mm |
Mobile phase: |
A. ACN : H2O (5 mM K2HPO4, 3 mM KH2PO4, pH7.5) = 125: 875; B. ACN : H2O : H3PO4 = 499: 499: 2 (v/v) |
Time (min): |
0 13 28 38 |
B (%): |
0 0 100 0 |
Flow rate: |
1.2 1.2 1.5 1.2 (mL/min) |
Temperature: |
40 oC |
Detection: |
UV 360 nm |
Injection volume: |
20 µL |
Sample: |
1. Vitamin B12 (維生素B12) |